A Rapid Quantitation of Occupational Aeroallergens by Magnetic Immuno-Chromatographic Test (MICT)

Dilek Inekci, Nicklas Skjoldager, Merete Simonsen, André Bergman, Fan Dong, Bjarne Vincents

Abstract

Quantitative point-of-use assay platforms for the simple, rapid and accurate detection of aeroallergens in non-laboratory settings are fundamental to investigate their aerodynamics and implement preventive interventions for aeroallergen-associated diseases. Here, we demonstrate the application of the novel assay platform Magnetic Immuno-Chromatographic Test (MICT) for the quantitation of fungal alpha-amylase in one step and with a total assay time of 30 minutes. MICT is a magnetic-based lateral flow immunoassay (LFIA) integrated into a lab-on-a-chip format and here developed with a novel built-in hook alert function. The superparamagnetic nanoparticle probes and the magnetic detection technology makes MICT superior over traditional chromophore-based LFIAs and allow a sensitivity and accuracy comparable with central laboratory immunoassays. The alpha-amylase MICT was adjusted to a dynamic range of 0.1 to 22.3 ng/mL showing an accuracy at 99.6% (%CV 9.5). The strong analytical performance of MICT in aeroallergen studies was demonstrated by spiking air sampling filters and performing air sampling trials and the results were compared with ELISA. The principal setup shows a novel, transferable and flexible approach for quantitation of various occupational allergens. The MICT offers the required functionalities to become the new versatile lab-on-a-chip tool for hazard prevention and control of occupational allergy and asthma by allowing continuous surveillance of the allergen levels in the working environment.

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